Post-Doctoral position – Nicolas Giraud group Université Paris Cité

NMR STUDIES OF SUPRAMOLECULAR INTERACTIONS INVOLVING AMYLOID PEPTIDES AND POLYPHOSPHATES
Biomolecular self-assembly is a timely question with broad impact from the development of materials to human health. The auto-catalytic self-assembly (routinely called aggregation) of peptides into amyloid fibrils is one of the most studied process in this field, during which partially unfolded monomers begin to interact together to ultimately form highly ordered supramolecular architectures. Within the context of amyloid-related diseases (e.g. Alzheimer disease), we aim at understanding the supramolecular assembly of amyloid-β (Aβ) peptides in the presence of polyanions such as polyphosphates (PP), a class of biologically relevant fibrils promoting agents. The interactions of PP with the Aβ peptides were shown to tune their self-assembly into fibrils, in link with literature data reporting a lessening of oligomers-mediated toxicity.
In this context, NMR is a well suited tool for studying the supramolecular interaction between Aβ peptides and PP. Together with Christelle Hureau in Toulouse, and Sébastien Blanchard,[1] we have developed along the last years a broad panel of hybrid methods involving NMR[2] to access the complete overview of Aβ peptides fibrillation mediated by PP. We will use state-of-the-art techniques in NMR combined with MD[3-4] to determine key structural and  dynamic features of the supramolecular interaction between Aß and PP. We will also probe the structure of PP and their interaction with fibrils using solid-state NMR (SS-NMR) 31P or 13C experiments conducted on fibrils samples analyzed at high field (700 MHz) at the Sorbonne University NMR facility with our collaborator Thierry Azaïs.
1. Atrian-Blasco, Hureau et al. Chem. Commun., 2022 https://doi.org/10.1039/D1CC05792H
2. Denis-Quanquin et al. Chem. Eur. J. 2016 https://dx.doi.org/10.1002:chem.201603460
3. Denis-Quanquin, Giraud et al. PCCP 2021 https://doi.org/10.1039/D0CP06570F
4. Dos Santos, Giraud et al. PCCP 2024 https://doi.org/10.1039/D4CP00463A
We are looking for a motivated postdoctoral fellow to join our consortium. The candidate will be in charge of (i) preparing NMR samples, (ii) implementing and optimizing NMR experiments, (iii) analyzing the resulting data, together with Molecular Dynamics results performed by our collaborator. He/she will be located at LCBPT at Université Paris Cité, a biomedical research center of excellence located downtown Paris, France. He/she will also have the opportunity to carry out Solid-State NMR experiments in Thierry Azaïs’ lab at LCMCP at Sorbonne University, Paris, one of the leader scientific university in France.
The NMR facilities at Université Paris Cité and Sorbonne University are located in the heart of Paris and provide a dynamic and international working environment. Paris is a unique and multicultural city, offering unequalled possibilities for cultural activities. It is served by 2 international airports and a dense high-speed rail network.
The eligible candidate will be funded for 18 months by a grant from the French Research Ministry (ANR grant SUPRAMY). Depending on the experience of the candidate, the net salary will be ~ 2200-2400 €/month.
Interested candidates (biochemists, chemists or biophysicists) should send a cv and a motivation letter to nicolas.giraud@u-paris.fr