Reminder: Post-doctoral position in biomolecular DNP-enhanced solid-state NMR @ Univ. Grenoble Alpes / CEA, France

We are still looking for a highly motivated candidate to work with Sabine Hediger and Gaël De Paëpe on developing DNP-enhanced solid-state NMR for the study of proteins in cells. The project will be done in collaboration with the group of Aurélien Deniaud, at CEA Grenoble, expert in cell biology, copper homeostasis in mammals, and biochemistry of copper binding proteins.

Context and scientific objectives of the project: We propose to solve the problems of resolution, sensitivity and selectivity associated with in-cell DNP experiments by developing an innovative approach based on the combination of bio-orthogonal chemistry and selective DNP (SelDNP). SelDNP is a methodology developed over the last few years in our group. It enables the production of high-resolution DNP-enhanced NMR subspectra specific to a predefined region of the protein, determined by the position of a spin label. Demonstrated so far only on purified proteins, the methodology is very promising for targeting a biomolecule in the crowded environment of the cell. The in-cell methodology will be developed and applied on mammalian cells for the study of Atox1, a copper (Cu) chaperone involved in Cu homeostasis.

Requirements: The candidate will work mainly in NMR and DNP, but depending on his/her expertise in biochemistry, he/she may assist our collaborator in protein production and labeling. Applicants are expected to have a doctoral degree in liquid-state and/or solid-state biomolecular NMR spectroscopy. Knowledge about MAS-DNP will be considered as a plus.

Application deadline: 31/05/2024

How To Apply: Send the application including CV, cover letter and references to sabine.hediger@cea.fr

Funding : The successful candidate will be recruited for 18 months, on a grant by the Labex Arcane (https://arcane.univ-grenoble-alpes.fr), a research program from the University Grenoble Alpes.

Scientific environment: The involved laboratories are research units of the Interdisciplinary Research Institute of Grenoble (IRIG), which brings together fundamental research in biology, health, nanoscience, cryotechnology and new technologies for energy and the environment. The DNP-NMR solid-state group (https://nmr-dnp-grenoble.net/home/) focuses on developing DNP-NMR instrumentation and methodology to push back the limits of the technique's sensitivity and resolution, in order to tackle challenging systems ranging from materials science to life sciences. The group of Aurélien Deniaud, at CEA Grenoble (https://www.cbm-lab.fr/en/Pages/MetOr/Presentation.aspx) is expert in cell biology, copper homeostasis in mammals, and biochemistry of copper binding proteins.

Local environment: Grenoble is one of the major cities in Europe for research with a large international scientific community. In addition, Grenoble has a large international student population, is a very pleasant city to live in, and is known as the “Capital of the Alps” with easy access to great skiing and hiking. It is also only 2 hours’ drive to the Mediterranean Sea, Italy, or Switzerland. Grenoble, Lyon, and Geneva airports are nearby and permit straightforward international travel.

-- _____________________________________________________________   Sabine Hediger Interdisciplinary Research Institute of Grenoble CEA Grenoble 17 rue des Martyrs 38054 Grenoble Cedex 9 France Tel.: +33 4 38 78 65 79 Fax : +33 4 38 78 50 90 Email : sabine.hediger@cea.fr _____________________________________________________________