Post-doc position : NMR of supramolecular interactions between amyloid peptides and inorganic polyanions

Dear colleagues,

We are looking for a hard-working and motivated postdoctoral fellow to join our consortium in the frame of an ANR-granted project (SUPRAMY).

Within the context of amyloid-related diseases, we aim at understanding the supramolecular assembly of amyloid-β (Aβ) peptides in the presence of polyanions, such as the biologically relevant polyphosphate polymers (PP) and the synthetic PolyOxoMetalates (POM). The interactions of such polyanions (P) with the peptides were shown to tune their self-assembly, in link with literature data reporting a lessening of oligomers-mediated toxicity.

The postdoctoral fellow will study the supramolecular interaction between Aβ peptides and P by combining NMR spectroscopy and Molecular Dynamics approach. In this frame, he/she will probe the structure of PP and their interaction with fibrils using 31P and 13C solid-state NMR (SS-NMR) experiments conducted on fibrils samples analyzed at high field (700 MHz-1 GHz). Solution NMR investigation will also be carried out to study the interaction at molecular level.

Our consortium gathers 4 researchers: Nicolas Giraud (Université Paris Cité), Christelle Hureau, (Coordination Chemistry Lab., Toulouse), Sébastien Blanchard and Thierry Azaïs (Sorbonne University). We develop an integrative approach to explore the chemistry and biophysics of amyloid-forming peptides and their interactions.

The candidate will be in charge of (i) preparing NMR samples, (ii) implementing and optimizing solution and solid/state NMR experiments, (iii) analyzing the resulting data, together with Molecular Dynamics results performed by our collaborator. He/she will be located at LCBPT at Université Paris Cité, a biomedical research center of excellence located downtown Paris, France, and will also work in Thierry Azaïs’ lab at Sorbonne Université for the SS-NMR experiments.

The NMR facilities at Université Paris Cité and Sorbonne University are located in the heart of Paris and provide a dynamic and international working environment:
• Université Paris Cité: our platform includes a state-of-the-art 600 MHz IVDr spectrometer fully dedicated for the analysis of biological samples and a spectrometer operating at 500 MHz, with a liquid-state cryoprobe optimized for 13C detection.
• Sorbonne University: 700, 500 and 300 MHz NMR spectrometers with solid-state probes are available, including a 1.3 mm Fast-MAS probe on the 700 MHz spectrometer.
• The Infranalytics network will be solicited to access very fast MAS probes (0.7 mm) at very high field (1 GHz) (https://infranalytics.fr/utilisateurs/plateformes).

Applicants should have a Ph.D. and a strong experience in biomolecular NMR (solution and/or solid-state). A specific focus on amyloid peptides will be particularly appreciated. Experience in molecular modeling of the investigated systems including interaction processes would be an advantage. Any citizenship is welcome, but applications will be evaluated by a Defence Security Official prior to any approval. The eligible candidate will be funded for 18 months by a grant from the French Research Ministry (ANR grant SUPRAMY), with a possible 6-month extension.

References:
1.      Dumont et al. PCCP 2013 https://dx.doi.org/10.1039/C3CP53671H
2.      Denis-Quanquin et al. Chem. Eur. J. 2016 https://dx.doi.org/10.1002:chem.201603460
3.      Denis-Quanquin et al. PCCP 2021 https://doi.org/10.1039/D0CP06570F
4.      Atrian-Blasco et al. Chem. Commun., 2022,58, 2367-2370 DOI https://doi.org/10.1039/D1CC05792H

Contact:
If you are interested in this position, please send CV, letter of interest and contact information for two/three references to Prof. Nicolas Giraud (nicolas.giraud@u-paris.fr)
Website : https://lcbpt.biomedicale.parisdescartes.fr/nmr

Best regards,
Nicolas Giraud.


Prof. Nicolas Giraud
Université Paris Cité

First year Program Chair, Master Frontiers in Chemistry
https://master-frontiers-in-chemistry.fr

Head, Research Group on NMR of Biological Substances
https://lcbpt.biomedicale.parisdescartes.fr/nmr

Faculty of Sciences
UFR Sciences Fondamentales et Biomédicales
LCBPT - UMR CNRS 8601
45 Rue des Saints-Pères, 75006 Paris

====================================